学术论文

脂质是生命不可或缺的生物分子，广泛参与膜构建、能量储存与信号传导。细胞中数以千计的脂质分子统称脂质组，浓度跨度达8个数量级，结构复杂且异构体共存。结构脂质组学旨在突破传统方法无法区分脂质异构体的局限，在精细结构层面为脂质组提供系统解决方案。

本课题组致力于发展异构体分辨串联质谱、异构体平行定量方法，及低丰度脂质组的选择性富集技术，推动脂质分子深度表征与生物医学应用。我们初步建立了光标记结合异构体分辨串联质谱的方法，揭示跨膜多肽诱导的膜脂疏水失配结构细节、脂质组成偏好及化学计量信息。未来，该方法有望拓展至膜蛋白体系，为理解脂质如何调控膜蛋白构象、稳定性与功能提供关键工具。

1. 脂质多层级结构的表征方法。建立了覆盖20多类脂质的PB-MS/MS分析方法以及双键位置异构体定性定量策略;开发了基于巯基自由基催化的脂质C=C顺反异构化技术，结合在线PB衍生，实现了脂质C=C构型（Z/E）及位置的双重表征。此外，我们还发展了新型自由基诱导解离（RDD）串联质谱方法，实现了对甘油磷脂sn-异构体定性定量、甲基支链以及羟基位点进行深度定性定量解析。 （Reviews links: Acc. Chem. Res. 2021, 54, 3873−3882；J. Lipid Res.2022, 63, 100219 ; Anal. Chem. 2025, 97, 4275 ; Anal. Chim. Acta, 2025, 344456）

2. 低丰度脂质选择性富集及分析方法。发展了基于磁性二氧化钛纳米材料（TiO₂ MNP）靶向鞘糖脂组的选择性富集方法，将检测动态范围提高1-3个数量级，绘制了脑组织、人血浆及乳腺癌细胞中超过1000个鞘糖脂分子的多层级结构图谱。

3. 高覆盖、深度脂质组分析流程的建立及生物医学应用。发展了光化学微流反应器（商业转化产品名：Omega Analyzer），建立了耦合液相色谱分离、在线PB衍生和串联质谱的脂质分析流程；进一步耦合离子淌度（TIMS）发展了具有数据自动解析能力的磷脂组深度分析系统。实现对细胞（1e5个）、组织（10 mg）、血清（20 µL）、人皮脂中上千种不饱和脂质分子实现双键位置鉴定和位置异构体定量。鞘糖脂组深度分析实现人神经胶质瘤亚型分型，揭示神经酰胺合成酶（CerS2）、脂肪酸二羟化酶（FA2H）在人胶质瘤组织中的显著下调。人肝细胞癌组织的深度磷脂分析揭示磷脂酰胆碱酰基转移酶1（LPCAT1）具有sn-1位置选择性。（Nat. Commun. 2019, 10, 79; Nat. Commun. 2023, 14, 4263；Angew. Chem. Int. Ed.2023, 62, e202215556）

4. 脂质-蛋白相互作用研究 。建立了模型多肽-膜脂疏水相互作用的表征方法。设计并合成了新型光交联探针，捕获了多肽跨膜区氨基酸残基与磷脂脂肪酸链的交联产物；发展了交联产物的液相色谱-串联质谱分析方法，实现不同交联位点分布的表征。与哈尔滨工业大学何元政团队合作及中科院上海药物所徐华强团队合作，鉴定多个膜蛋白的脂质配体精细结构。

54. Zidan Wang, Yichun Wang, Yu Xia*, Multi-structural profiling of neutral glycosphingolipids using selective enrichment and the Paternò–Büchi derivatization, Methods Enzymol. 2026: 357-378.

53. Lipeng Qiao, Jing Zhao, and Yu Xia*, Isobaric Intramolecular Paternò–Büchi Reactions Enable Multiplexed Quantitation of Fatty Acids down to C═C Locations, J. Am. Chem. Soc.  2026, 148 (41): 44629–44638.

52. Yichun Wang#, Gaoge Sun#, Hang Yin* and Yu Xia*, Deep Profiling Reveals Coordinated Remodeling of Ganglioside Metabolism in Breast Cancer Cell Line, Chem. Sci., 2026, 17, 6488–6499

51. Xue Zhao, Hengxue Shi, Qiaohong Lin, Yu Xia*, Review: Deep lipidomic profiling enabled by the Paternò-Büchi reaction – Pinpointing C=C locations and beyond, Anal. Chim. Acta, 2025, 344456.

50. Hengxue Shi, Huijiao Bai, Haiteng Deng, Yu Xia*, Revealing Acyl Chain Selectivity of Cis-to-Trans Isomerase through Profiling of C═C Geometry and Location Isomers of Bacterial Lipids, Anal. Chem., 2025, 97, 10378–10387.

49. Y. Liu, Y. Xia*, W. Zhang*, Structural Lipidomics Enabled by Isomer-Resolved Tandem Mass Spectrometry, Anal. Chem., 2025, 97, 4275-4286.

48.Y. Xiong#, Z. Xu#, X. Li#, Y. Wang#, J. Zhao#, N. Wang, Y. Duan, R. Xia, Z. Han, Y. Qian, J. Liang, A. Zhang, C. Guo, A. Inoue, Y. Xia*, Z. Chen*, Y. He*, Identification of oleic acid as an endogenous ligand of GPR3, Cell Res., 2024, 34, 232-24

47. Z. Wang, D. Zhang, J. Wu, W. Zhang, Y. Xia*, “Illuminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels”, Nat. Commun., 2024, 15, 5627

46. Z. Wang, Y. Xia*, “Selective Enrichment via TiO2 Magnetic Nanoparticles Enables Deep Profiling of Circulating Neutral Glycosphingolipids”, Anal. Chem., 2024, 96, 16955−16963

45.Z. Chen*, Y., Gong, F. Chen, H.J. Lee, J. Qian, J. Zhao, W. Zhang, Y. Li, Y. Zhou, Q. Xu, Y. Xia, L. Zhou*, J-X Cheng*, Orchestrated desaturation reprogramming from stearoyl-CoA desaturase to fatty acid desaturase 2 in cancer epithelial-mesenchymal transition and metastasis. Cancer Commun.. 2024;

44. Yichun Wang · Yu Xia. Deep profiling of plasmalogens by coupling the Paternò-Büchi derivatization with tandem mass spectrometry. Anal Bioanal Chem. 2024;416(19):4397-4407.

doi:10.1007/s00216-024-05376-9

43. Hengxue Shi  Yu Xia. Shotgun Lipidomic Profiling of Sebum Lipids via Photocatalyzed Paternò-Büchi Reaction and Ion Mobility-Mass Spectrometry. Anal Chem. 2024 ;96(14):5589-5597.

doi:10.1021/acs.analchem.4c00141

42. Tian Xia, Xue Jin, Donghui Zhang, Jitong Wang, Ruijun Jian, Hang Yin, Yu Xia*, "Alternative fatty acid desaturation pathways revealed by deep profiling of total fatty acids in RAW 264.7 cell line", J. Lipid. Res. 2023.

https://doi.org/10.1016/j.jlr.2023.100410

41.  Qiaohong Lin, Ruijun Jian, Shengzhuo Wang, Yu Xia*, "Characterization of Oxidized Glycerophosphoethanolamines via Radical-Directed Dissociation Tandem Mass Spectrometry and the Paternò–Büchi Derivatization", Anal. Chem. 2023, 95, 25, 9422–9427.

https://doi.org/10.1021/acs.analchem.3c00792

40.  Tian Xia, Feng Zhou, Donghui Zhang, Xue Jin, Hengxue Shi, Hang Yin, Yanqing Gong, Yu Xia*, "Deep-profiling of phospholipidome via rapid orthogonal separations and isomer-resolved mass spectrometry", Nat. Commun., 2023, 14, 4263.

https://doi.org/10.1038/s41467-023-40046-x

39.  Hengxue Shi, Zhenshu Tan, Xiangyu Guo, Hanlin Ren, Shengzhuo Wang, Yu Xia*, "Visible-Light Paternò–Büchi Reaction for Lipidomic Profiling at Detailed Structure Levels", Anal. Chem. 2023, 95, 11, 5117–5125.

https://doi.org/10.1021/acs.analchem.3c00085

38.  Wenpeng Zhang, Ruijun Jian, Jing Zhao, Yikun Liu, Yu Xia*, "Deep-lipidotyping by mass spectrometry: recent technical advances and applications", Journal of Lipid Research, 2022.

https://doi.org/10.1016/j.jlr.2022.100219

37.  Donghui Zhang, Qiaohong Lin, Tian Xia, Jing Zhao, Wenpeng Zhang, Zheng Ouyang* and Yu Xia*, "LipidOA: A Machine-Learning and Prior-Knowledge-Based Tool for Structural Annotation of Glycerophospholipids", Anal. Chem. 2022, 94, 48, 16759–16767.

https://doi.org/10.1021/acs.analchem.2c03505

36. Hai-Fang Li, Jing Zhao, Wenbo Cao, Wenpeng Zhang, Yu Xia*, and Zheng Ouyang*, “Site-Specific Photochemical Reaction for Improved C=C Location Analysis of Unsaturated Lipids by Ultraviolet Photodissociation” Research, 2022, Article ID 9783602, Published: 12 Feb 2022

https://doi.org/10.34133/2022/9783602

35. Xiaoxiao Ma, Wenpeng Zhang, Zishuai Li, Yu Xia* and Zheng Ouyang*, Enabling High Structural Specificity to Lipidomics by Coupling Photochemical Derivatization with Tandem Mass Spectrometry. Acc. Chem. Res. 2021, 54, 20, 3873–3882.

https://doi.org/10.1021/acs.accounts.1c00419

34. Zishuai Li, Simin Cheng, Qiaohong Lin, Wenbo Cao, Jing Yang, Minmin Zhang, Aijun Shen, Wenpeng Zhang, Yu Xia, Xiaoxiao Ma* and Zheng Ouyang*, "Single-cell lipidomics with high structural specificity by mass spectrometry" Nature Communications, 2021, 12, 2869.

https://doi.org/10.1038/s41467-021-23161-5

33. Qiaohong Lin, Pengyun Li, Mengxuan Fang, Donghui Zhang, and Yu Xia*, “Deep Profiling of Aminophospholipids Reveals a Dysregulated Desaturation Pattern in Breast Cancer Cell Lines” Anal. Chem. 2021, Publication Date:December 21

https://doi.org/10.1021/acs.analchem.1c03494

32. Jing Zhao, Mengxuan Fang, Yu Xia*, “A Liquid Chromatography-Mass Spectrometry Workflow for In-Depth Quantitation of Fatty Acid Double Bond Location Isomers”J. Lipid. Res. 2021, Available online 24 August .

https://doi.org/10.1016/j.jlr.2021.100110

31. Qingyuan Hu, Yu Xia*, Xiaoxiao Ma*, “Comprehensive Structural Characterization of Lipids by Coupling Paternò–Büchi Reaction and Tandem Mass Spectrometry”, In: Hsu FF. (eds) Mass Spectrometry-Based Lipidomics. Methods in Molecular Biology, vol 2306. Humana, New York, NY.

https://doi.org/10.1007/978-1-0716-1410-5_4

30. Tian Xia, Ming Yuan, Yongwei Xu, Feng Zhou, Kate Yu*, and Yu Xia*, “Deep Structural Annotation of Glycerolipids by the Charge-Tagging Paterno–Büchi Reaction and Supercritical Fluid Chromatography–Ion Mobility Mass Spectrometry", Anal. Chem. 2021, 93, 23, 8345–8353

https://pubs.acs.org/doi/10.1021/acs.analchem.1c01379

29. Hanlin Ren, Alexander Triebl, Sneha Muralidharan, Markus R. Wenk*, Yu Xia* and Federico Torta*, "Mapping the Distribution of Double Bond Location Isomers in Lipids across Mouse Tissues", Analyst, 2021,146, 3899-3907

https://doi.org/10.1039/D1AN00449B

28. X. Ma, Y. Xia, "Unsaturated Lipid Analysis via Coupling the Paternò–Büchi Reaction with ESI-MS/MS", Lipidomics. 2020: 148-174.

27. Xue Zhao, Gang Wu, Wenpeng Zhang, Mengqiu Dong, and Yu Xia*, "Resolving Modifications on Sphingoid Base and N-Acyl Chain of Sphingomyelin Lipids in Complex Lipid Extracts", Anal. Chem. 2020, 92, 21, 14775–14782

https://doi.org/10.1021/acs.analchem.0c03502

26. Jing Zhao, Xiaobo Xie, Qiaohong Lin, Xiaoxiao Ma, Pei Su, Yu Xia*, "Next-Generation Paternò–Büchi Reagents for Lipid Analysis by Mass Spectrometry", Anal. Chem. 2020, 92, 19, 13470–13477

https://pubs.acs.org/doi/10.1021/acs.analchem.0c02896

25. Elissia T. Franklin, Yu Xia*, "Structural elucidation of triacylglycerol using online acetone Paternò–Büchi reaction coupled with reversed-phase liquid chromatography mass spectrometry" , Analyst. 2020, 145, 6532-6540.

https://doi.org/10.1039/D0AN01353F

24. Elissia Franklin, Samuel Shields, Jeffrey Manthorpe, Jeffrey C. Smith, Yu Xia, Scott A. Mcluckey*, "Coupling Headgroup and Alkene Specific Solution Modifications with Gas-Phase Ion/Ion Reactions for Sensitive Glycerophospholipid Identification and Characterization", J. Am. Soc. Mass Spectrom. 2020, 31, 4, 938–945.

https://doi.org/10.1021/jasms.0c00001

23. Wenpeng Zhang*, Bing Shang, Zheng Ouyang, Yu Xia*, "Enhanced Phospholipid Isomer Analysis by Online Photochemical Derivatization and RPLC-MS", Anal. Chem. 2020, 92, 9, 6719–6726.

https://doi.org/10.1021/acs.analchem.0c00690

22. Tian Xia, Hanlin Ren, Wenpeng Zhang, Yu Xia*, "Lipidome-Wide Characterization of Phosphatidylinositols and Phosphatidylglycerols on C=C Location Level", Analytica Chimica Acta, 2020, 1128, 107-115.

http://dx.doi.org/10.1016/j.aca.2020.06.017

21. Wenbo Cao, Simin Cheng, Jing Yang, Wenpeng Zhang, Zishuai Li, Qinhua Chen, Yu Xia, Zheng Ouyang*, Xiaoxiao Ma*, "Large-scale lipid analysis with C=C location and sn-position isomer resolving power", Nat Commun, 2020, 11, 375.

https://www.nature.com/articles/s41467-019-14180-4

20. 马潇潇，胡清源，瑕瑜*, "Paternò-Büchi(PB)反应与串联质谱结合实现不饱和脂质精确结构解析", 分析测试学报, 2020, 39(1), 19-27.

http://new.fxcsxb.com/fxcsxb/ch/reader/view_abstract.aspx?file_no=20200103&flag=1

19. Xiaobo Xie, Jing Zhao, Miao Lin, Jinlan Zhang, Yu Xia*, "Profiling of Cholesteryl Esters by Coupling Charge Tagging Paternò-Büchi Reaction and Liquid Chromatography-Mass Spectrometry", Anal. Chem. 2020, 92, 12, 8487–8496.

https://doi.org/10.1021/acs.analchem.0c01241

18. Haifang Li, Wenbo Cao, Xiaoxiao Ma, Xiaobo Xie, Yu Xia, Zheng Ouyang*, "Visible-Light-Driven [2 + 2] Photocycloadditions between Benzophenone and C=C Bonds in Unsaturated Lipids", J. Am. Chem. Soc. 2020, 142, 7, 3499–3505.

https://doi.org/10.1021/jacs.9b12120

17. Wenpeng Zhang, Bing Shang, Yu Xia, "Comprehensive Characterization of Phospholipid Isomers in Human Platelets", J. Anal. Test., 2020, 4, 210–216.

https://link.springer.com/article/10.1007/s41664-020-00137-w

16. Q. Lin, D. Zhang, Y. Xia*, "Analysis of Ether Glycerophosphocholines at the Level of C=C Locations from Human Plasma", Analyst, 2020, 145, 513-522.

https://doi.org/10.1039/C9AN01515A

15. X. Zhao, W. Zhang, D. Zhang, X. Liu, W. Cao, Q. Chen, Z. Ouyang, Y. Xia*, "A Lipidomic Workflow Capable of Resolving sn- and C=C Location Isomers of Phosphatidylcholines", Chem. Science, 2019, 10, 10740-10748.

https://doi.org/10.1039/C9SC03521D

14. Y. Su, J. Page, X. Ma, R. Shi, Y. Xia*, Zheng Ouyang*, "Mapping Lipid C=C Location Isomers in Organ Tissues by Coupling Photochemical Derivatization and Rapid Extractive Mass Spectrometry", Int. J. Mass Spectrom. 2019, 445, 116206

https://doi.org/10.1016/j.ijms.2019.116206

13. Elissia T. Franklin, Stella K. Betancourt, Caitlin E. Randolph, Scott A. McLuckey* , and Yu Xia* ,"In-depth structural characterization of phospholipids by pairing solution photochemical reaction with charge inversion ion/ion chemistry",  Anal Bioanal Chem. 2019, 411, 4739–4749.

https://doi.org/10.1007/s00216-018-1537-1

12. R. Zou, W. Cao, L. Chong, W. Hua, H. Xu, Y. Mao, J. Page, R. Shi, Y. Xia, Tony Y. Hu, W. Zhang*, and Z. Ouyang*, "Point-of-Care Tissue Analysis Using Miniature Mass Spectrometer", Anal. Chem. 2019, 91, 1, 1157–1163

https://doi.org/10.1021/acs.analchem.8b04935

11. X. Xie, Y. Xia*, "Analysis of Conjugated Fatty Acid Isomers by the Paternò-Büchi Reaction and Trapped Ion Mobility Mass Spectrometry", Anal. Chem. 2019, 91, 7173-7180.

https://doi.org/10.1021/acs.analchem.9b00374

10. W. Zhang, S. Chiang, Z. Li, Q. Chen, Y. Xia, Z. Ouyang*, "A Polymer Coating Transfer Enrichment for Direct Mass Spectrometry Analysis of Lipids in Biofluid Samples", Angew. Chem., Int. Ed., 2019, 58, 6064-6069.

https://doi.org/10.1002/anie.201900011

9. W. Zhang, D. Zhang, Q. Chen, J. Wu, Z. Ouyang*, Y. Xia*, "Online photochemical derivatization enables comprehensive mass spectrometric analysis of unsaturated phospholipid isomers", Nat. Commun., 2019, 10, 79.

https://www.nature.com/articles/s41467-018-07963-8

8. L. Chong, R. Tian, R. Shi, Z. Ouyang*, and Y. Xia*, "Coupling the Paternò-Büchi (PB) Reaction With Mass Spectrometry to Study Unsaturated Fatty Acids in Mouse Model of Multiple Sclerosis", Front. Chem. 2019, 7:807.

https://doi.org/10.3389/fchem.2019.00807

7. F. Tang*, C. Guo, X. Ma, J. Zhang, Y. Su, R. Tian, R. Shi, Y. Xia, X. Wang, Z. Ouyang*, "Rapid in situ Profiling of Lipid C=C Location Isomers in Tissue Using Ambient Mass Spectrometry with Photochemical Reactions", Anal. Chem. 2018, 90, 5612-5619

https://doi.org/10.1021/acs.analchem.7b04675

6. J. Li, S. Condello, J. T. Pepin, X. Ma, Y. Xia, T. D. Hurley, D. Matei*, and J. Cheng*, “Lipid Desaturation Is a Metabolic Marker and Therapeutic Target of Ovarian Cancer Stem Cells”, Cell Stem Cell, 2017, 20, 3, 301-314

https://doi.org/10.1016/j.stem.2016.11.004

5. J. Ren, E.T. Franklin, Y. Xia*, "Uncovering Structural Diversity of Unsaturated Fatty Acyls in Cholesteryl Esters via Photochemical Reaction and Tandem Mass Spectrometry", J. Am. Soc. Mass Spectrom. 2017, 28, 1432-1441

https://doi.org/10.1021/jasms.8b05584

4. X. Ma, X. Zhao, J. Li, W. Zhang, J.-X. Cheng, Z. Ouyang*, Y. Xia*, "Photochemical Tagging for Quantitation of Unsaturated Fatty Acids by Mass Spectrometry", Anal. Chem. 2016, 88, 8931–8935

https://doi.org/10.1021/acs.analchem.6b02834

3. C. A. Stinson, Y. Xia*, "A Method of coupling Paternò-Büchi reaction with direct infusion ESI-MS/MS for locating C=C bond in glycerophospholipids", Analyst, 2016, 141, 3696-3704

https://doi.org/10.1039/C6AN00015K

2. X. Ma, L. Chong, R. Tian, R. Shi, T. Y. Hu, Z. Ouyang*, Y. Xia*, "Identification and quantitation of lipid C=C location isomers: a shotgun lipidomics approach enabled by photochemical reaction", Proc. Natl. Acad. Sci. USA, 2016, 113, 2573-2578. Featured by C&E News and Nature Methods

https://doi.org/10.1073/pnas.1523356113

1. X. Ma and Y. Xia*, "Pinpointing Double Bonds in Lipids by Paternò–Büchi Reactions and Mass Spectrometry", Angew. Chem., Int. Ed, 2014, 53, 2592-2596.

https://doi.org/10.1002/anie.201310699

75. Andrea Cerrato; Aldo Laganà; Carmela Maria Montone; Enrico Taglioni; Daniel Weissman; Anna Laura Capriotti. Aza-Paternò–Büchi derivatization enables annotation of triacylglycerols by negative-ion-mode mass spectrometry at the double bond level. Talanta  2027, 312(Pt A), 130396. Published online 7 August  2026. https://doi.org/10.1016/j.talanta.2026.130396

74. Xi Chen; Gopal Reddy Ramidi; Nitya Shree; Dallas Freitas; Syuan-Ting Kuo; Huiying Guo; Luchen Wuyang; Aidan Slagter; Peiyu Cai; Ashok Polu; Mahua Choudhury; Xin Yan. Duplex Paternò–Büchi Isobaric Reagents for One-Step C═C-Resolved Lipid Isomer Quantification. Journal of the American Chemical Society  2026, 148(39), 42508–42520. https://doi.org/10.1021/jacs.6c13813

73. Ruoling Yang; Man Song; Qingyang Liu; Jingwen Tang; Yujun Wei; Dongxue Ge; Kun Qian; Yangliu Shao; Haoyang Zhang; Hanlin Xue; Yikun Liu; Liping Dou; Daihong Liu. Structural lipidomics identifies murine plasma C=C location isomers for radiation dose stratification. Cell Biology and Toxicology 2026. Published online 28 August  2026. https://doi.org/10.1007/s10565-026-10257-1

72. Yiwei Tou; Wei Sun; Pai Liu; Jiuyi Sun; Taiyang Ma; Yun-Hong Zhang. Structural Lipidomics Uncovers C═C Location-Specific Lipid Signatures and the Response to Immune Checkpoint Inhibitor in dMMR/MSI-H Colorectal Cancer. ACS Measurement Science Au  2026, 6(3), 655–667. https://doi.org/10.1021/acsmeasuresciau.5c00200

71. Andrea Cerrato; Enrico Taglioni; Chiara Cavaliere; Aldo Laganà; Elena Lucà; Carmela Maria Montone; Anna Laura Capriotti. Azetidination-based photocycloaddition enables regioisomer-resolved annotation of cholesteryl esters by negative-ion-mode LC-MS/MS. Analytica Chimica Acta  2026, 1402, 345392. https://doi.org/10.1016/j.aca.2026.345392

70. Wanying Hu; Wenhan Li; Meirong Song; Jianfei Zhu; Kui Zhu. Rule-Based Ion Prediction with Orthogonal Constraints Reveals Bacterial Phospholipid Remodeling Signatures. Antibiotics 2026, 15(5), 459. https://doi.org/10.3390/antibiotics15050459

69. Zhijun Cai; Ningxi Li; Simin Cheng; Zheng Ouyang; Xiaoxiao Ma. Single-cell structural lipidomics using a miniature dual-LIT mass spectrometer. Chemical Science  2026, 17(7), 3828–3840. https://doi.org/10.1039/D5SC08665E

68. Manal Alhusban; Suha Telfah; Mohammad M. Al-Gharaibeh; Sanaa Bardaweel; Raghad Alkadri; Fang Wei. Comprehensive Profiling of Triglycerides in Wild Eastern Mediterranean Echium Seed Oil Using Paternò–Büchi Modulated Lipidomics. Molecules 2026, 31(3), 550. https://doi.org/10.3390/molecules31030550

67. Zhuoning Xie; Qirui Yu; Simin Cheng; Dan Li; Zheng Ouyang; Xiaoxiao Ma. ¹³C-Isotope Tracing Structural Lipidomics for Resolving Phospholipid Metabolism Dynamics in Human Breast Cancer Cells. Analytical Chemistry  2026, 98(3), 2401–2413. https://doi.org/10.1021/acs.analchem.5c06789

66. Yang Gu; Ming-Ming Shao; Song-Ping Cui; Bin Hu; Xin Li. Deep structural lipidomic profiling reveals C=C positional isomers as potential biomarkers in lung adenocarcinoma tissue. Translational Lung Cancer Research  2025, 14(11), 4768–4783. https://doi.org/10.21037/tlcr-2025-717

65. Hao Xu; Tianhang Jiang; Yuxiang Lin; Lei Zhang; Huan Yang; Xiaoyun Huang; Ridong Mao; Zhu Yang; Changchun Zeng; Shuang Zhao; Lijun Di; Wenbin Zhang; Jun Zeng; Zongwei Cai; Shu-Hai Lin. LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomics. Nature Communications  2025, 16, 4566. https://doi.org/10.1038/s41467-025-59683-5